Publications & Media


Biotechnology Focus May 2012

Biotechnology Focus November 2012

Genetic Engineering & Biotechnology News  “Exploiting Cells’ Electrical Behavior” (Sept.2013)


Aghamohseni H, Ohadi K, Spearman M, Krahn N, Moo-Young M, Scharer J, Butler M, Budman H. 2014. Effects of nutrient levels and average culture pH on the glycolysis pattern of camelid-humanized monoclonal antibody. J. Biotechnol. 186:98. PDF

Bodnar E, Perreault H. 2013. Qualitative and quantitative assessment on the use of magnetic nanoparticles for glycopeptide enrichment. Analytical Chemistry. 85:10895. PDF

Bodnar E, Nascimento T, Girard L, Komatsu E, Lopez P, Gomes de Oliveira A, Roy R, Smythe T, Zogbi Y, Spearman M, et al. 2015. An integrated approach to analyze EG2-hFc monoclonal antibody N-glycosylation by MALDI-MS. Can J Chem. 93:754.

Bodnar E, Perreault H. 2015. Synthesis and evaluation of carboxymethyl chitosan for glycopeptide enrichment. – Analytica Chimica Acta. 891:179.

Bodnar E, Perreault H. 2013. Qualitative and Quantitative Assessment on the Use of Magnetic Nanoparticles for Glycopeptide Enrichment. Analytical Chemistry. 85:10895.

Bodnar E, Raymond C, Lopez P, Villacrés C, Butler M, Schoenhofen I, Durocher Y, Perreault H. 2015. Mass spectrometric analysis of products of metabolic glycan engineering with azido-modification of sialic acids. Anal Bioanal Chem.

Braasch K, Nikolic-Jaric M, Cabel T, Salimi E, Bridges GE, Thomson DJ, Butler M. 2013. The changing dielectric properties of CHO cells can be used to determine early apoptotic events in a bioprocess. Biotechnology and Bioengineering. 110:2902. PDF

Butler M, Meneses-Acosta A. 2012. Recent advances in technology supporting bippharmaceutical production from mammalian cells. Appl Microbiol Biotechnol. 96:885-894.

Butler M, Spearman M. 2014. The choice of mammalian cell host and possibilities for glycosylation engineering. Curr Opin Biotechnol. 30:107. PDF

Butler M. 2013. From the mold in dr Florey’s coat to blockbuster drugs. Pharm. Bioprocess. 1:3. PDF

Butler M. 2013. Serum-free media: Standardizing cell culture systems. Pharm. Bioprocess. 1:1. PDF

Butler M, Spearman M, Braasch K. 2013. Monitoring cell growth, viability, and apoptosis, p. 169. In Poertner R (ed.), AnimalCellBiotechnology-MethodsandProtocols, 3rd ed., vol. 1104. Humana Press.PDF

Céline Raymond, Anna Robotham, John Kelly, Erika Lattová, Hélène Perreault and Yves Durocher. 2012. Production of highly sialylated monoclonal antibodies , p. 397. In Stefana Petrescu (ed.), Glycosylation. InTech.

Dorion-Thibaudeau J, Raymond C, Lattová E, Perreault H, Durocher Y, De Crescenzo G. 2014. Towards the development of a surface plasmon resonance assay to evaluate the glycosylation pattern of monoclonal antibodies using the extracellular domains of CD16a and CD64. J. Immunol. Methods. 408:24-34.PDF

Dorion-Thibaudeau J, St-Laurent G, Raymond C, De Crescenzo G, Durocher Y. 2015. Biotinylation of the Fc? receptor ectodomains by mammalian cell co-transfection: application to the development of a surface plasmon resonance-based assay. Journal of Molecular Recognition:n/a-n/a.

Ghorbaniaghdam A , Chen J, Henry O, Jolicoeur M. 2014. Analyzing clonal variation of monoclonal antibody-producing CHO cell lines using an in silico metabolomic platform. Plos One. E 9(3): e90832PDF

Ghorbaniaghdam A, Henry O, Jolicoeur M. 2013. A kinetic-metabolic model based on cell energetic state: Study of CHO cell behavior under na-butyrate stimulation. Bioprocess Biosyst Eng. 36:469. PDF

Ghorbaniaghdam A, Henry O, Jolicoeur M. 2014. An in-silico study of the regulation of CHO cells glycolysis. Journal of Theoretical Biology. 357:112-122. PDF

Gomes de Oliveira AG, Roy R, Raymond C, Bodnar ED, Tayi VS, Butler M, Durocher Y, Perreault H. 2015. A systematic study of glycopeptide esterification for the semi-quantitative determination of sialylation in antibodies. Rapid Communications in Mass Spectrometry. 29:1817-1826.

Hassel KJ, Moresoli C. 2016. Role of pH and Ionic strength on weak cation exchange macroporous Hydrogel membranes and IgG capture. J Membr Sci. 498:158-166.

Ho R, McConkey BJ. 2011. Modulating glycosylation of recombinant monoclonal antibodies for therapeutic applications. Current Topics in Biotechnologyvol. 6: 57-69.

Kim DY, Chaudhry MA, Kennard ML, Jardon MA, Braasch K, Dionne B, Butler M, Piret JM. 2013. Fed-Batch CHO Cell t-PA Production and Feed Glutamine Replacement to Reduce Ammonia Production. Biotechnol Prog. 29:165.

Jardon M, Sattha B, Braasch K, Leung A, Côté H, Butler M, Gorski S, Piret J. 2012. Inhibition of glutamine-dependent autophagy increases t-PA production in CHO cell fed-batch processes. Biotechnol. Bioeng. 109:1228-1238.

Kim DY, Chaudhry MA, Kennard ML, Jardon MA, Braasch K, Dionne B, Butler M, Piret JM. 2013. Fed-batch CHO cell t-PA production and feed glutamine replacement to reduce ammonia production. Biotechnol. Prog. 29:165.PDF

Lattova E, Perreault H. 2013. The usefulness of hydrazine derivitives for mass spectrometric analysis of carbohydrates. Mass Spectrometry Reviews. 32:366. PDF

Lin S, Jan JT, Dionne B, Butler M, Huang M, Wu C, Wong C, Wong S. 2013. Different immunity elicited by recombinant H5N1 hemagglutinin proteins containing pauci-mannose, high-mannose, or complex type N-glycans. Plos One. 8. PDF

Lui B, Spearman M, Doering J, Lattova E., Perreault H, Butler M. 2013. The availability of glucose to CHO cells affects the intracellularlipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody. J. Biotech. 6571:1. PDF

Naderi S, Nikdel A, Meshram M, McConkey B, Ingalls B, Budman H, Scharer J. 2014. Modeling of cell culture damage and recovery leads to increased antibody and biomass productivity in CHO cell cultures. Biotechnology Journal. 9:1152-1163.

Nasseri SS, Ghaffari N, Braasch K, Jardon MA, Butler M, Kennard M, Gopaluni B, Piret JM. 2014. Increased CHO cell fed-batch monoclonal antibody production using the autophagy inhibitor 3-MA or gradually increasing osmolality. Biochem. Eng. J. 91:37-45. PDF

Nikolic-Jaric M, Cabel T, Salimi E, Bhide A, Braasch K, Butler M, Bridges GE, Thomson DJ. 2013. Differential electronic detector to monitor apoptosis using dielectrophoresis-induced translation of flowing cells (dielectrophoresis cytometry). Biomicrofluidics. 7:1. PDF

Ohadi K, Legge RL, Budman HM. 2015. Development of a soft-sensor based on multi-wavelength fluorescence spectroscopy and a dynamic metabolic model for monitoring mammalian cell cultures. Biotechnol Bioeng. 112:197-208.

Perreault H, Lattová E, Šagi D, Peter-Katalinic J. 2013. Carbohydrates and glycolipids. In Hillenkamp F, Peter-Katalinic J (eds.), MALDI-MS: A Practical Guide to Instrumentation, Methods and Applications, Second Edition ed., . Wiley-VCH Verlag GmbH & Co., Weinheim, Germany.

Raymond C, Robotham A, Spearman M, Butler M, Kelly J, Durocher Y. 2015. Production of α2,6-sialylated IgG1 in CHO cells. mAbs. 7:3:571.

Raymond C, Robotham A, Kelly J, Lattová E, Perreault H., Durocher Y. 2012. Production of highly sialylated monoclonal antibodies, p. 397. In Stefana Petrescu (ed.), Glycosylation. InTech.

Rizi BS, Braasch K, Salimi E, Bridges G, Thompson D. 2014. Monitoring the dielectric response of single cells following mitochondrial adenosine triphosphate synthase inhibition by oligomycin using a dielectrophoretic cytometer. Biomicrofluidics. 8:4114.

Robitaille J, Chen J, Jolicoeur M. 2015. A Single Dynamic Metabolic Model Can Describe mAb Producing CHO Cell Batch and Fed-Batch Cultures on Different Culture Media. PLoS ONE:e0136815.

Sadavarte R, Spearman M, Okun N, Butler M, Ghosh R. 2014. Purification of chimeric heavy chain monoclonal antibody EG2-hFc using hydrophobic interaction membrane chromatography; An alternative to protein-A affinity chromatography. Biotechnol Bioeng. 111:1139-1149.

Sadvarte R, Ghosh R. 2014. A thermal-cycling method for disaggregating monoclonal antibody oligomers. Journal Of Pharmaceutical Sciences. 103:870. PDF

Sokolenko S, Blondeel EJM, Azlah N, George B, Schulze S, Chang D, Aucoin MG. 2014. Profiling Convoluted 1D-1H-NMR Spectra: A Plackett-Burman Approach for Assessing Quantification Error of Metabolites Complex Mixtures with Application to Cell Culture. Analytical Chemistry. 86 (7): 3330-3337.

Sokolenko S, George S, Wagner A, Tuladhar A, Andrich J, Aucoin M. 2012. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks. Biotechnol Adv. 30(3):766-781.

Sokolenko S, McKay R, Blondeel EJ, Lewis MJ, Chang D, George B, Aucoin MG. 2013. Understanding the variability of compound quantification from targeted profiling metabolomics of 1D-1H-NMR spectra in synthetic mixtures and urine with additional insights on choice of pulse sequences and robotic sampling. Metabolomics. 9:887. PDF

Spearman M, Bodnar E, Perreault H, Butler M. 2014. Glycosylation analysis of chinese hamster ovary produced glycoproteins. Pharmaceutical Bioprocesses. 2: 449.

Spearman M, Dionne B, Butler M. 2011. The role of glycosylation in therapeutic antibodies, p. 251. In Al-Rubeai M (ed.), Antibody Expression and Production Cell Engineeringvol. 7. Springer Netherlands.

Spearman M, Lattova E, Perreault H., Butler M. 2013. Glycolsylation. In Zhou W, Komives C (eds.), Novel Bioprocessing Technology for the Production of Biopharmaceuticals and Bioproducts. John Wiley & Sons Inc.

Tayi V, Butler M. 2013. Physiology and metabolism of animal cells for production. In Wagner A, Hauser H (eds.), Mammalian Cell Biotechnology in Biologics Production. DeGruyter.

Tayi V.; Butler, M. 2015. Isolation and quantification of N-glycans from immunoglobulin G antibodies for quantitative glycosylation analysis. Journal of Biological Methods. 2(2) e.19.  PDF

Yen S, Sokolenko S, Manocha B, Patras A, Daynouri-Pancino F, Blondeel E, Sasges M, Aucoin MG. 2014. Treating Cell Culture Media with UV Irradiation against Adventitious Agents: Minimal Impact on CHO Performance. Biotechnology Progress. 30(5):1190–1195.

Yoo SM, Ghosh R. 2012. Simultaneous removal of leached protein-A and aggregates from monoclonal antibody using hydrophobic interaction membrane chromatography. Journal of Membrane Science. 390-391:263-269.